Office: Clapp 115
Molecular biology of human cytomegalovirus (HCMV) replication.
Current research goals:
1) To characterize the glycoprotein complexes present in the HCMV envelope that mediate attachment and entry.
2) To define the mechanisms by which HCMV enters different types of cells.
3) To better understand the specific roles of epithelial and endothelial cells in the pathogenesis of HCMV disease.
1) Virology (BIOM 435) 3 CR Spring semesters.
2) Advanced Virology (BIOM 509) 3CR Fall semester, even-numbered years.
3) Topics of Virology (BIOB 596) 1CR Spring semesters.
2003-2009 Postdoctoral Training, Oregon Health and Science, Portland, OR
2003 Ph.D. University of Iowa, Iowa City, IA
1997 B.S., Winona State University, Winona, MN
Zhou M, Yu Q, Wechsler A, Ryckman BJ. (2013) Comparative Analysis of gO Isoforms Reveals that Strains of Human Cytomegalovirus Differ in the Ratio of gH/gL/gO and gH/gL/UL128-131 in the Virion Envelope. J. Virol. 87:9680-9690.
Ryckman,B.J., M.C. Chase, and D.C. Johnson. (2010) Human cytomegalovirus TR strain glycoprotein O acts as a chaperone promoting gH/gL incorporation into virions but is not present in virions. J. Virol. 84:2597-2609
Ryckman,B.J., M.C. Chase, and D.C. Johnson. (2008) HCMV gH/gL/UL128-131 interferes with virus entry into epithelial cells: evidence for cell type-specific receptors. Proc. Natl. Acad. Sci. 105:14118-14123.
Ryckman, B.J., B. Rainish, M.C. Chase, J. Borton, M.A. Jarvis, J.A. Nelson and D.C. Johnson. (2008) Characterization of the HCMV gH/gL/UL128-131 complex that mediates entry into epithelial and endothelial cells. J. Virol. 82:60-70.
Ryckman, B.J., M.A. Jarvis, D.D. Drummond, J.A. Nelson and D.C. Johnson. (2006) Human cytomegalovirus entry into epithelial and endothelial cells depends on genes UL128 to UL150 and occurs by endocytosis and low-pH fusion. J. Virol. 80:710-722.